Key resources table.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| pACL ser454/455 | Cell signaling technologies | CS43315 |
| ACL | Thermo Fisher | PA5-29495 |
| pAKT ser473 | Cell signaling technologies | 9271 |
| AKT | Cell signaling technologies | 9272 |
| PPM1K | Abcam | Ab135286 |
| GFP | Clontech | 632375 |
| BDK | Santa Cruz | sc374425 |
| V5 | Genetex | GTX-42525 |
| p-e1a BCKDH ser293 | Abcam | ab200577 |
| e1a BCKDH | Santa Cruz | sc-67200 |
| GAPDH | Sigma | G8795 |
| B-tubulin | Sigma | T5326 |
| ETFA | Abcam | ab110316 |
| COXIV | Li-Cor | 926-42214 |
| Bacterial and Virus Strains | ||
| Ad-CMV-BDK | In house | N/A |
| Ad-CMV-Bgal | In house | N/A |
| Ad-CMV-GFP | Vector Biolabs | 1060 |
| Ad-CMV-PPM1K | Vector Biolabs | ADV-219587 |
| Ad-CMV-ChREBP | In house | N/A |
| Ad-CMV-GFP (control for ChREBP) | In house | N/A |
| Ad-CMV-ΔMTS-BDK | In house | N/A |
| CMV-ΔMTS-BDK-GFP | In house | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| BT2, 3,6-Dichlorobenzo[β]thiophene-2-carboxylic acid | Sigma | Discontinued we have recently validated BT2 from Chem-Impex Int. INC Cat# 25643 |
| GST-tagged human ACL | Sigma | SRP0288 |
| 40 KDa subunit of bovine PKA | Promega | V5161 |
| Critical Commercial Assays | ||
| Rat Insulin ELISA | EMD Millipore | EZRMI-13K |
| V5-tagged protein purification Kit | MBL | 3317 |
| TransIT-293 Transfection Reagent | Mirus | MIR2700 |
| Image-IT Live Mito and Nuclear Labeling Kit | ThermoFisher | I34154 |
| Triglyceride | Abcam | Ab65336 |
| 6-plex Tandem Mass Tag Kit | ThermoFisher | 90061 |
| iScript cDNA synthesis kit | BioRad | 1708890 |
| TRI reagent | MRC | Tr118 |
| Deposited Data | ||
| Raw LC-MS/MS data | ProteomeXchange Consortium via Pride partner repository | PXD009122 |
| Experimental Models: Cell Lines | ||
| Fao hepatoma cells | Sigma | 85061112 |
| Hek293 cells | ATCC | CRL-1573 |
| Experimental Models: Organisms/Strains | ||
| Wistar rats | Charles River Laboratories | Strain:003 |
| Zucker Fatty Rats | Charles River Laboratories | Zuc-FA/Fa Strain:185 |
| Oligonucleotides | ||
| See Supplemental Table 2 | ||
| Recombinant DNA | ||
| Gateway pDONR223 BDK plasmid | DNASU | HsCd00511364 |
| Gateway pDONR223 ACL plasmid | DNASU | HsCd00399238 |
| pAd/CMV/V5-DEST Bgal plasmid | Thermolife | 49320 |
| Software and Algorithms | ||
| Proteome Discoverer v2.1 | ThermoFisher | |
| PhosphoSitePlus® | www.phosphosite.org | |
| PRIDE partner repository accession number | ProteomeXchange | PXD009122 |