Fig. 6.

Effect of chronic dosing with ATX-MS-1467 on in vivo proinflammatory cytokine secretion. (DR2 × Ob1)F1 mice received from 1 to 10 treatments with 100 µg of ATX-MS-1467 or vehicle (PBS) on days 0, 2, 5, 7, 9, 12, 14, 16, 18 and 20. The top panel shows the overall dosing schedule (a). Serum cytokine concentrations were assayed 2 h after the last injection. The bottom panel shows the absolute concentrations of each cytokine in serum (b–e) used to generate ratios of interleukin 10 versus the other cytokines, shown in the insert (f–h). Data were analyzed by ANOVA followed by Dunnett’s multi-comparison test. *, **, *** and **** indicate p < 0.05, < 0.01, < 0.001 and < 0.0001, respectively, versus the vehicle (PBS)-treated group. ANOVA analysis of variance, IFN interferon, IL interleukin, PBS phosphate-buffered saline