Fig. 7.

Effect of chronic treatment with ATX-MS-1467 on the ratio of interleukin 10-to-proinflammatory cytokines after an acute challenge with myelin basic protein. The length of the wash-out period is indicated by the arrows in the schematic representation of the study design (a). The absolute serum cytokine concentrations (b–e) were used to generate ratios of interleukin 10 versus the other cytokines shown in the insert (f–h). Data were analyzed by ANOVA followed by Dunnett’s multicomparison test. *, **, *** and **** indicates p < 0.05, < 0.01, < 0.001 and < 0.0001, respectively, versus naïve mice (first column). ^#, ^##, ^### and ^#### indicate p < 0.05, < 0.01, < 0.001 and < 0.0001, respectively, versus mice that were challenged with MBP without any prior tolerization (HLAbp column only), n = 6–8. ^†After 10 doses of ATX-MS-1467. ANOVA analysis of variance, HLAbp human leukocyte antigen-binding peptide, IFN interferon, IL interleukin, MBP myelin basic protein, na not applicable, PBS phosphate-buffered saline