Fig. 8.

Effect of chronic treatment with ATX-MS-1467 on the splenic iTreg population of (DR2 × Ob1)F1 mice. For a–c, the spleens were harvested and fresh cells stained as described in the methods section. a, b are representative plots showing the populations of LAG3⁺CD49b⁺ cells (CD3⁺CD8⁻B220⁻) from total CD4⁺ lymphocytes, for which the averages are shown in c. For d–g, the harvested splenocytes were stimulated in vitro for 3 h in the presence of PMA, ionomycin and monensin, to allow intracellular accumulation and subsequent staining of IL-10. The contributions of Foxp3⁻ and Foxp3⁺ cells to the total IL 10-producing CD4⁺ lymphocytes is shown in d and e, which are representative dot plots of the groups of mice treated with HLAbp (n = 8) or ATX-MS-1467 (n = 8), respectively. The averaged data are shown in f and g. * and ** indicate p < 0.05 and < 0.01, respectively, by unpaired t test with Welch’s correction versus the HLAbp group (n = 8). Foxp3 forkhead box p3, HLAbp human leukocyte antigen-binding peptide, IL interleukin, iTreg induced regulatory T cells, PMA phorbol myristate acetate