Table 3.

Antibody panels used for splenocyte staining

Panel 1 (cell surface markers on freshly isolated splenocytes)	Panel 2 (cell surface and intracellular markers)
CD49b (BV421, 1:100, cat. # 63063, BD Pharmingen, San Jose, CA, USA)	CD49b (BV421, 1:100, cat. # 63063] BD Pharmingen, San Jose, CA, USA)
CD3 (V500, 1:100, cat. # 560771, BD Pharmingen, San Jose, CA, USA)	CD4 (V500, 1:100, cat. # 560782] BD Pharmingen San Jose, CA, USA)
CD4 (FITC, 1:100, cat. # 553729, BD Pharmingen, San Jose, CA, USA)	LAG3 (PE, 1:100, cat. # 125207, Biolegend, San Diego, CA, USA)
LAG3 (PE, 1:100, cat. # 125207, Biolegend, San Diego, CA, USA)	IL-10 (APC, 1:100, cat. # 554468, BD Pharmingen, San Jose, CA, USA)
B220 (PE-Cy7, 1:200, cat. # 552772, BD Pharmingen, San Jose, CA, USA)	CD8 (APC-Cy-7, 1:100, cat. # NC0079947, Biolegend, San Diego, CA, USA)
CD8 (BV605, 1:100, 563152, cat. # 563152, BD Pharmingen, San Jose, CA, USA)	CD45R/B220 (BV605 1:100, cat. # 563708, BD Pharmingen, San Jose, CA, USA)
FC block (CD16/CD32, unstained, 1:100, cat. # 553141, BD Pharmingen, San Jose, CA, USA)	FC block (CD16/CD32, unstained, 1:100, cat. # 553141, BD Pharmingen, San Jose, CA, USA)
	FoxP3 (PerCP-Cy5.5 1:100, cat. # 35-5773-82, eBioscience, San Diego, CA, USA)

CD45R/B220 and CD8 staining were used as a negative selection for the T lymphocyte subpopulations of interest. Listed are the specificities, fluorochromes, catalogue numbers and manufacturers, and dilutions for each of the antibodies