Fig. 3.

Growth profile (a, d), viability (b, e), and titre (c, f) of GS-CHO cells grown as fed-batch culture in the micro-Matrix at a shaking speed of 280 rpm, a working volume of 3 mL, and active control of temperature (37 °C), DO (30%), and pH (7.2). In run 1 (a–c) no compensation for evaporation was performed, whereas run 2 (d–f) was subjected to this procedure five times. Arrows indicate the time points of dH₂O additions to counteract evaporation based on the sodium level measured