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Endpoint values of the relative liquid loss (a) and osmolality (b) after 15 days of two fed-batch cultivations with GS-CHO cells grown in the micro-Matrix. In run 1 no compensation for evaporation was performed, whereas run 2 was subjected to this procedure five times. The shaking speed for both runs was set to 280 rpm, the working volume was 3 mL, temperature was controlled at 37 °C, DO at 30%, and pH at 7.2
