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. 2018 May 31;9:577. doi: 10.3389/fphar.2018.00577

FIGURE 3.

FIGURE 3

Open channel block of hERG by FHND004. (A) Representative current elicited by the “envelope of tails” protocol before and after application of 10 μM FHND004. Cells were held at a holding potential of -80 mV and pulsed to depolarizing voltage (+30 mV) for variable durations from 20 to 1420 ms in 100 ms increments. Envelope tail currents were recorded upon repolarization to -40 mV. (B) Mean plots of current density against development of depolarizing time fitted with single exponential equation before and after the application of 10 μM FHND004 (n = 6). (C) A plot of the onset of current block expressed as relative tail current [relative tail current = (currents with drug/currents without drug) × 100%]. The time-dependent decay in relative tail current was fitted to a mono-exponential equation (n = 6). (D) Representative current traces recorded before and after the addition of 10 μM FHND004, using the protocol shown in the inset (n = 5).