Table 1.
Antibodies used with product information.
| Antibody | Made in species | Vendor | Product number | Dilution | Antigen retrieval? | Primary or secondary |
|---|---|---|---|---|---|---|
| Iba1 | Goat | Abcam | ab5076 | 1:2000 | No | Primary |
| CD68 | Rat | Biorad | MCA1957 | 1:500 | No | Primary |
| BrdU | Rat | Abcam | OBT0030 | 1:500 | 2N HCl at 37°C for 30 min | Primary |
| DCX | Goat | Santa Cruz Biotechnologies | SC-8066 | 1:500 | No | Primary |
| NeuN | Mouse | Millipore | MAB377 | 1:1000 | No | Primary |
| Biotin anti-rat | Donkey | Jackson ImmunoResearch | 102649–782 | 1:500 | N/A | Secondary |
| AlexaFluor647 anti-goat | Donkey | Invitrogen | A21447 | 1:500 | N/A | Secondary |
| AlexaFluor594 anti-rat | Donkey | Invitrogen | A21209 | 1:500 | N/A | Secondary |
| Streptavidin AlexaFluor555 | N/A | Invitrogen | S21381 | 1:1000 | N/A | Tertiary |