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. 2018 May 31;9:661. doi: 10.3389/fphys.2018.00661

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Copyright © 2018 Guillén, Platas, Pérez del Caz, Mirabet and Alcaraz.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phagocytosis in human macrophages. Phagocytosis of fluorescent beads was determined after 3 h. (A) Quantitative analysis (mean ± SD from 4 separate experiments with cells from different donors), ++P < 0.01 compared to control (B: nonstimulated cells); ^∗∗P < 0.01 compared to F1 or F2, as appropriate. (B) Representative fluorescent images. CD45-FITC (green), DAPI (blue), fluorescent beads (red). Concentrations of fluorescent beads: F1, 10⁷/ml and F2, 5 × 10⁷/ml. Microscopic magnification of the objective lens 20 × . Bar = 50 μm.