Skip to main content
. 2018 May 31;9:1235. doi: 10.3389/fimmu.2018.01235

Figure 2.

Figure 2

Lack of RhoA expression prevents T-cell infiltration in brain parenchyma. (A) Representative micrograph of a sagittal brain section from a RhoAfl/flLckCre− mouse, stained for CD3. Magnification of the brain section from a RhoAfl/flLckCre− mouse (B) and a RhoAfl/flLckCre+ mouse (C), where CD3+ T-cells can be observed in the brain parenchyma (black arrows) and the perivascular space (red arrows). (D) Quantification by IHC of the CD3+ T-cells present in the cerebral area of the brain section from RhoAfl/flLckCre− (n = 33) and RhoAfl/flLckCre+ (n = 24) brain sections. Data are represented as number of CD3+ T-cells per area (mm2). (E–K) Quantification by flow cytometry of CD4+ T-cells (E), IFNγ+ T-cells (F), TNFα+ T-cells (G), GM-CSF+ T-cells (H), IL-17A+ T-cells (I), IL-4+ T-cells (J), and CD11b+ cells (K) present in spinal cord of experimental autoimmune encephalomyelitis mice (n = 3 per group). Data shown as mean ± SEM. *p < 0.05, **p < 0.01 (unpaired t-test).