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. 2018 May 31;9:1235. doi: 10.3389/fimmu.2018.01235

Figure 5.

Figure 5

RhoA is important in T-cell transendothelial migration. (A) Quantification of migratory capacity of RhoA+/+ and RhoA−/− T-cells across an endothelium, analyzed by live-cell time lapse imaging. Data representative of two independent experiments. (B) Representative images of migrated RhoA+/+ (left) and RhoA−/− (right) T-cells at the bottom of the migration chamber after 4 h of migration. Marked in orange are the migrated cells detected and quantified by the software IncuCyte™ Basic Software (C). Gating strategy used for flow cytometric analysis of migrated T-cells. (D) Quantification of the total number of migrated cells after 4 h by flow cytometry. Data represent the mean of two independent experiments. Data shown as mean ± SEM *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 [(A) Two-way ANOVA, followed by Sidak’s test. (D) Unpaired t-test].