Figure 2.

Insulin effect was mediated through PI3K/Akt pathway. (A) In slices previously incubated with the PI3K inhibitor, LY294002 (1 μM) and insulin, gabazine had no effect on the tonic current. (B) The incubation with insulin and the inactive analog of the PI3K inhibitor, LY303511 (5 μM) failed to prevent the shift of the tonic current induced by gabazine. (C) In slices incubated with insulin and genistein (50 μM), a tyrosine kinase inhibitor, gabazine produced a small effect on the tonic current. (D) Box plot summary showing the normalized shift produced by gabazine for LY294002 (n = 5), LY303511 (n = 7), genestein (n = 6), and wortmannin (200 nM, n = 5) ^*p < 0.05, ^**p < 0.01.