Figure 3.

Insulin-sensitive tonic current is mediated by α5 and δ subunit-containing GABA_A receptors. (A) The α5 inverse agonist, L-655, 708 antagonizes GABA_A receptor-mediated tonic current in pyramidal neurons. Note that gabazine application caused an additional shift of the tonic current (upper dash line). (B) Shift of the tonic current by L-655, 708 and the subsequent application of gabazine expressed as percentage of the basal level (n = 7). The inset shows a box plot comparing L-655, 708 with L-655, 708 plus gabazine (GBZ). (C) THIP (5 μM) an agonist for δ subunit-containing GABA_A receptors, induces a downward shift in the holding current which is then blocked by gabazine application. (D) Shift of the tonic current by THIP and gabazine application expressed as percentage of the basal level (n = 5). The inset shows a box plot summary of the results. These experiments were performed in slices incubated with insulin (20 nM) ^*p < 0.05.