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The viability of Hep-2 cells treated by PPD, PCL/3%PPD, PCL/5%PPD, PCL/10%PPD and PCL/20%PPD at an equivalent PPD dose of 20 µg ml−1, 30 µg ml−1 and 40 µg ml−1 for 1 (a), 3 (b) and 5 (c) days. (d) Annexin V/PI analysis of the apoptosis of Hep-2 cells treated with an equivalent PPD dose of 20 µg ml−1 after 3 days. The data are presented as average ± standard deviation (n = 5). Statistical significance: *p < 0.05; **p < 0.01.
