Table 1.
protein | predicted pI | molecular weight (kDa) | hydropathicity (GRAVY) | expression strain | yield (mg l−1)a |
---|---|---|---|---|---|
ELP[KEY4-n] | |||||
n = 24 | 6.38 | 15.5 | −0.321 | BL21(DE3)pLysS | 62 |
n = 48 | 6.39 | 26.6 | −0.209 | Rosetta2(DE3)pLysS | 358 |
n = 96 | 6.40 | 48.8 | −0.140 | BL21(DE3) | 47 |
ELP[K2Y2V2-48] | 10.11 | 25.5 | 0.095 | BL21-CodonPlus-(DE3)-RIPL | 71 |
ELP[K3Y3-48] | 10.23 | 26.3 | −0.295 | BL21(DE3)pLysS | 17 |
aYield was calculated per litre of bacterial culture.