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. 2018 May 9;5(5):171225. doi: 10.1098/rsos.171225

Table 1.

Detailed information on recombinant proteins examined in this study.

protein predicted pI molecular weight (kDa) hydropathicity (GRAVY) expression strain yield (mg l−1)a
ELP[KEY4-n]
n = 24 6.38 15.5 −0.321 BL21(DE3)pLysS 62
n = 48 6.39 26.6 −0.209 Rosetta2(DE3)pLysS 358
n = 96 6.40 48.8 −0.140 BL21(DE3) 47
ELP[K2Y2V2-48] 10.11 25.5 0.095 BL21-CodonPlus-(DE3)-RIPL 71
ELP[K3Y3-48] 10.23 26.3 −0.295 BL21(DE3)pLysS 17

aYield was calculated per litre of bacterial culture.