TABLE 2 .
Yeast strains used in this study
| Strain | Genotype | Source or reference |
|---|---|---|
| W303α | MATα 15ade2-1 ura3-1,112 his3-11 trp1 leu2-3 | This study |
| NKY39 | MATa 15ade2-1 ura3-1,112 his3-11 trp1 leu2-3 VIIL::ADE2 CHL1-13MYC::TRP | This study |
| NKY40 | MATa 15ade2-1 ura3-1,112 his3-11 trp1 leu2-3 VIIL::ADE2 HSC82::KANr CHL1-13MYC::TRP | This study |
| NKY41 | MATa 15ade2-1 ura3-1,112 his3-11 trp1 leu2-3 VIIL::ADE2 HSP82::KANr CHL1-13MYC::TRP | This study |
| NKY43 | MATα 15ade2-1 ura3-1,112 his3-11 trp1 leu2-3 CHL1-13MYC::TRP Δpdr::loxP-leu2-loxP | This study |
| NKY2 | MATα 15ade2-1 ura3-1,112 his3-11 trp1 leu2-3 CHL1-13MYC::TRP | This study |
| NKY3 | MATα 15ade2-1 ura3-1,112 his3-11 trp1 leu2-3 chl1::TRP | This study |
| SLY89 |
Δhsc82::kanMX4Δhsp82::kanMX4/piHGpd-G170Dhsp82-HIS Δpdr::loxP-leu2-loxP
trp1-289 leu2-3,112 his3-Δ200URA 3-52 ade2-101Δc lys2-801 am |
This study |
| NKY45 |
Δhsc82::kanMX4Δhsp82::kanMX4/piHGpd-G170Dhsp82-HIS Δpdr::loxP-leu2-loxP trp1-289
leu2-3,112 his3-Δ200URA 3-52 ade2-101Δc lys2-801 am CHL1-13MYC::TRP |
This study |
| 7D MATa |
7D MATa
SPC29-CFP::KAN mRFP-TETR URA3::TETO
GFP-LACI::LEU2 YCPlac112GAL CEN LACO-TRP1 |
Gift from Santanu K. Ghosh |
| NKY4 | 7D MATa SPC29-CFP::KAN mRFP-TETR URA3::TETO GFP-LACI::LEU2 YCPlac112GAL | This study |
| NKY9 |
7D MATa
SPC29-CFP::KAN mRFP-TETR URA3::TETO GFP-LACI::LEU2 YCPlac112GAL PDS1-13MYC::TRP |
This study |
| NKY61 |
7D MATa
SPC29-CFP::KAN mRFP-TETR URA3::TETO GFP-LACI::LEU2 YCPlac112GAL PDS1-13MYC::TRP pdr5::HIS3 |
This study |
| NKY62 |
7D MATa
SPC29-CFP::KAN mRFP-TETR URA3::TETO GFP-LACI::LEU2 YCPlac112GAL chl1::HIS3 PDS1-13MYC::TRP |
This study |
| YMH58a | MATa 15ade2-1 ura3-1,112 his3-11 trp1-1 leu2-3 CFIII (CEN3.L.YMH58) URA3 SUP11 | Gift from Akash Gunjan |
| NKY46 | MATa 15ade2-1 ura3-1,112 his3-11 trp1-1 leu2-3 CFIII (CEN3.L.YMH58) URA3 SUP11 chl1::TRP | This study |
| NKY47 | MATa 15ade2-1 ura3-1,112 his3-11 trp1-1 leu2-3 CFIII (CEN3.L.YMH58) URA3 SUP11 pdr5::TRP | This study |
| SBY1 |
MATa
15ade2-1 ura3-1,112 his3-11 trp1-1 leu2-3 CFIII (CEN3.L.YMH58) URA3 SUP11 chl1::TRP pLAchl1-N-466 |
This study |
| SBY2 |
MATa
15ade2-1 ura3-1,112 his3-11 trp1-1 leu2-3 CFIII (CEN3.L.YMH58) URA3 SUP11 chl1::TRP pLACHL1 |
This study |
| SBY3 |
MATa
15ade2-1 ura3-1,112 his3-11 trp1-1 leu2-3 CFIII (CEN3.L.YMH58) URA3 SUP11 chl1::TRP pLA |
This study |
| PJ69-4A |
MATa
trpl-901 leu2-3,112 ura3-52 his3-200 ga14Δ ga180Δ LYS2:: GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ |
37 |
| NKY48 |
MATa
trpl-901 leu2-3,112 ura3-52 his3-200 ga14Δ ga180Δ LYS2:: GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ pGBDUC1 pGADC1 |
This study |
| NKY49 |
MATa
trpl-901 leu2-3,112 ura3-52 his3-200 ga14Δ ga180Δ LYS2:: GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ pGBDUC1/HSP82 pGADC1/CHL1 |
This study |
| NKY50 |
MATa
trpl-901 leu2-3,112 ura3-52 his3-200 ga14Δ ga180Δ LYS2:: GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ pGBDUC1/HSP82 pGADC1 |
This study |
| NKY51 |
MATa
trpl-901 leu2-3,112 ura3-52 his3-200 ga14Δ ga180Δ LYS2::GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ pGBDUC1 pGADC1/CHL1 |
This study |
| NKY52 |
MATa
trpl-901 leu2-3,112 ura3-52 his3-200 ga14Δ ga180Δ LYS2:: GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ pGBDUC1 pGADC1/chl1-N466 |
This study |
| NKY53 |
MATa
trpl-901 leu2-3,112 ura3-52 his3-200 ga14Δ ga180Δ LYS2:: GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ pGBDUC1 pGADC1/chl1-C648 |
This study |
| NKY54 |
MATa
trpl-901 leu2-3,112 ura3-52 his3-200 ga14Δ ga180Δ LYS2:: GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ pGBDUC1 pGADC1/chl1-C253 |
This study |
| NKY55 |
MATa
trpl-901 leu2-3,112 ura3-52 his3-200 ga14Δ ga180Δ LYS2:: GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ pGBDUC1 pGADC1/chl1-C170 |
This study |
| NKY56 |
MATa
trpl-901 leu2-3,112 ura3-52 his3-200 ga14Δ ga180Δ LYS2:: GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ pGBDUC1/HSP82 pGADC1/chl1-N466 |
This study |
| NKY57 |
MATa
trpl-901 leu2-3,112 ura3-52 his3-200 ga14Δ ga180Δ LYS2:: GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ pGBDUC1/HSP82 pGADC1/chl1-C648 |
This study |
| NKY58 |
MATa
trpl-901 leu2-3,112 ura3-52 his3-200 ga14Δ ga180Δ LYS2:: GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ pGBDUC1/HSP82 pGADC1/chl1-C253 |
This study |
| NKY59 |
MATa
trpl-901 leu2-3,112 ura3-52 his3-200 ga14Δ ga180Δ LYS2:: GAL1-HIS3 GAL2-ADE2 met2::GAL7-lacZ pGBDUC1/HSP82 pGADC1/chl1-C170 |
This study |