Table 1.
Cellular, biochemical parameters and inflammatory mediator in BALF and homogenates after 4 days, a month and 3 months from intratracheal instillation
| Groups | TiO2 NP (mg/kg) |
|||||||
|---|---|---|---|---|---|---|---|---|
| control | 0.5 | 1.5 | 5 | 15 | 50 | 150 | RLSD | |
| 4 days | ||||||||
| Cell viability% | 94.15±74 | 84.96±0.47*a | 75.91±1.83*b | 83.15±0.49*a | 81.94±1.42*b | 89.79±1.95*d | 87.18±1.57*d | 1.61 |
| BALF TNF-α (ng/L) | 130.33±11.31 | 97.33±15.5*a | 68.00±15.62*b | 86.67±12.02*a | 94.67±7.77*a | 94.33±22.62*a | 96.33±12.16*a | 6.73 |
| BALF MIP-2 (ng/L) | 81.67±8.48 | 147.67±26.53*a | 212.33±16.9*c | 138.33±18.38*a | 121.33±9.23*b | 141.67±18.61*a | 237.67±13.43*b | 5.83 |
| Homog TNF-α (ng/L) | 113.33±38.18 | 31.33±9.86*a | 54.67±22.03*a | 61.33±11.37*a | 57.33±26.87*a | 68.67±22.03*b | 40.00±3.46*a | 6.88 |
| Homog MIP-2 (ng/L) | 90.67±16.44 | 27.0±8.66*a | 54.67±10.61*b | 65.0±10.39*b | 64.67±7.09*b | 65.33±8.96*b | 50.33±4.24*b | 4.92 |
| 1 month | ||||||||
| Cell viability% | 98.82±0.087 | 92.77±0.25*a | 95.89±1.655*b | 95.71±1.339*b | 94.38±0.843*b | 95.096±0.616*b | 94.094±1.535*b | 1.71 |
| BALF TNF-α (ng/L) | 145.33±21.2 | 251.67±33.29*a | 216.33±16.52*a | 209.33±21.0*b | 244.0±39.34*a | 266.33±46.49*c | 241.67±46.51*a | 10.09 |
| BALF MIP-2 (ng/L) | 90.33±8.74 | 131.56±7.77*a | 129.67±9.19*a | 128.67±34.29*a | 128.33±20.21*a | 160.33±9.29*b | 135.56±14.85*a | 8.17 |
| Homog TNF-α (ng/L) | 115.67±5.67 | 339.33±31.39*a | 286.67±21.15*b | 286.0±21.63*b | 286.33±17.32*b | 323.0±42.03*a | 221.33±28.58*c | 26.28 |
| Homog MIP-2 (ng/L) | 88.33±8.74 | 136.56±16.26*a | 135.33±12.52*a | 130.33±32.71*a | 100.67±16.97*b | 130.56±24.82*a | 103.33±46.82*b | 10.51 |
| 3 months | ||||||||
| Cell viability% | 98.82±0.08 | 92.77±0.25*a | 95.08±1.25*b | 96.41±0.82*b | 94.38±0.84*c | 96.31±1.87*b | 94.09±1.53*c | 1.79 |
| BALF TNF-α (ng/L) | 144.33±25.42 | 262.67±15.04*a | 232.67±10.61a | 217.33±18.35*a | 215.0±21.23*a | 192.33±24.21*b | 225.33±35.35*a | 9.34 |
| BALF MIP-2 (ng/L) | 83.33±7.07 | 163.33±14.14*a | 132.33±5.65*b | 127.33±9.89*b | 140.33±13.31*c | 140.67±38.89*c | 127.33±6.93*b | 7.54 |
| Homog TNF-α (ng/L) | 116.33±12.5 | 121.0±12.5*a | 184.33±4.94*b | 140.0±42.21*a | 202.67±38.89*b | 225.67±4.24*b | 287.67±34.15*c | 7.83 |
| Homog MIP-2 (ng/L) | 81.33±9.19 | 153.33±18.23*a | 135.0±10.52*b | 129.67±14.2*b | 155.0±7.54*a | 137.67±38.89*b | 182.0±39.96*c | 8.11 |
Values are mean ± SD (n-3)
*
Represent a significant difference in comparison between treated groups and control (p<0.05)
a,b,c
The same letter means no significant between groups while different letters mean there was significant between groups