Table 2.
Summary of increased gut microbiota variations in biopsy sample of colorectal cancer patients
| Reference | Sample type | Increased bacteria | Method |
|---|---|---|---|
| Mima et al. 2016(49) | Tumor tissue samples | F. nucleatum | quantitative PCR assay |
| Wei et al. 2016(50) | Tumor tissue samples | F. nucleatum and Bacteroides fragilis | 16S rRNA gene pyrosequencing |
| Li et al. 2016(56) | Tumor tissue samples | F. nucleatum | FQ-PCR |
| Zhou et al. 2016(57) | Tumor tissue samples | Fusobacterium spp., E.faecalis, ETBF | Real-time PCR |
| Burns et al. 2015(46) | Tumor tissue samples | Fusobacterium and Providencia | qPCR and 16S rRNA gene pyrosequencing |
| Gao et al. 2015(47) | Tumor tissue samples | Firmicutes and Fusobacteria | 16S rRNA gene pyrosequencing |
| Mira-Pascual et al. 2015(48) | mucosal and fecal samples | F. nucleatum and Enterobacteriaceae | qPCR and 16S ribosomal RNA gene pyrosequencing |
| Viljoen et al. 2015(75) | CRC tissues |
fusobacterium spp., enterotoxigenic
Bacteroides fragilis (ETBF) |
Real-time PCR |
| Tahara et al. 2014 (76) |
CRC tissues | F. nucleatum and Pan-fusobacterium | Real-time PCR |
| Geng et al. 2013(77) | Tumor/matching normal tissue of Chinese CRC patients | Fusobacterium spp., Roseburia | pyrosequencing-based molecular monitoring of bacterial 16S rRNA gene |
| Warren et al. 2013(78) | CRC/matching normal tissues | Fusobacterium, Leptotrichia and Campylobacter | 16S rRNA gene pyrosequencing |
| Castellarin et al. 2012(14) | Tumor/matching normal tissues | Fusobacterium nucleatum | RNA sequencing |
| Kostic et al. 2012 (65) | Tumor/matching normal tissues | Fusobacterium | whole genome sequences and confirmed by quantitative PCR and 16S rDNA sequence |
| Marchesi et al. 2011 (20) | Tumor/matching normal tissues | Fusobacterium | rRNA sequencing |