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. 2018 Apr 18;90(11):6580–6586. doi: 10.1021/acs.analchem.8b00269

Figure 4.

Figure 4

Detection of standard LAMP and SD-LAMP reactions in pure water. Electrophoresis gels verifying amplification (top), LFIA test results (middle), and LFIA test line quantification (bottom). (A) Standard LAMP reaction products for V. cholerae labeled with primers yield false positive LFIA results in low concentration samples and no template control (NTC) reactions. (B) SD-LAMP reactions yield no false positive LFIA results for V. cholerae. n = 4, replicates indicated by each circle. *** indicates p ≤ 0.001; ** indicates p ≤ 0.01; * indicates p ≤ 0.05.