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Direct detection of MS2 in PBS solution without RNA extraction. Correlation analysis indicates significant linear relationship between direct gLAMP counts with traditional plaque assay counts (r2 = 0.984, p < 0.05). A similar relationship was also found between log10-transformed plaque assay counts and the Cq values of RT-qPCR (r2 = 0.994, p < 0.05). Error bars represent the standard deviation of triplicate independent experiments.
