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. 2018 May 3;90(11):6601–6609. doi: 10.1021/acs.analchem.8b00382

Figure 4.

Figure 4

Capillary HILIC-MS with online RPLC trap-column and CaptiveSpray ESI source of a mixture of cytochrome C and ribonuclease A (0.02 mg/mL each, 10 μL injection) using pure nitrogen (magenta), nitrogen with ACN as DEN-gas (brown), and ACN with 1% PA as DEN-gas (blue). (a) Total-ion chromatograms and (b) mass spectra of ribonuclease A (and respective most intense mass). isCID energy, 30 eV; trap-column loaded for 3 min at 15 μL/min; linear gradient from 10 to 20% B in 1 min, from 20 to 50% B in 10 min, from 50 to 90% B in 2 min.