Figure 1.

GEM increased HLA class I expression on tumour cells in a β2 m-associated manner. a) Representative histograms showing change in HLA-A,B,C MFI in response to culture with drugs at IC₂₅. b) The effect of chemotherapy drugs on surface expression of HLA-A,B,C on tumour cells as measured by flow cytometry. Data are expressed relative to untreated controls. Mean and standard deviation is plotted and values are significantly different (**** = p<0.0001, ** = p<0.01) to controls by one-way ANOVA with Dunnett's multiple comparisons test. n = 3. c) Blots representative of three experiments showing expression of HLA α-heavy chains and β2 m proteins in untreated (Un), IFNγ-treated (1000 IU/ml) or GEM-treated (100 nM) tumour cells. d) Transcription of the β2 m gene was increased in HCT116 cells in response to GEM, as assessed by qPCR. n = 1. e) Mean fold-change in mRNA for HLA class I genes in response to GEM. n = 3. f) Tumour cells transfected with human β2 m expressing plasmid had increased surface expression of HLA class I 48 hours after transfection, as measured by flow cytometry. Means and standard deviations are plotted and mock-transfected and β2 m-transfected are significantly different by student's paired t-test. For A549 and MCF-7, n = 3, for HCT116 n = 6.