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. 2018 Jun 6;38(23):5277–5288. doi: 10.1523/JNEUROSCI.3028-17.2018

Figure 3.

Figure 3.

Specific role of RARα in barrel cortex in texture preference and novel texture discrimination. A, Quantification of percentage of L5 PN expressing RARα mRNA measured by single-cell RT-qPCR of P30 WT and S1-RARα cKO mice (***p < 0.001, one-way ANOVA; n = the number of cells used from 3 mice per genotype). B, Representative images of P30 WT (mCRE) and S1-RARα cKO (CRE) cortices visualized by cytochrome c staining in tangential sections and quantification of S1 surface area outlined in images. Scale bar, 500 μm. C, Representative image of stereotaxic injection into S1 of RARαfl/fl mice of AAV virus expressing synapsin promoter driven active Cre or inactive mCRE. Scale bar, 500 μm. D, Quantification of texture preference in S1-RARα cKO mice. Bar graphs show percentage of time spent at texture 400 and 220 (left; ***p < 0.001, one-way ANOVA) and discrimination ratios of the two textures of S1-RARα cKO mice (right; **p < 0.01, one-way ANOVA). E, Quantification of Y-maze test in S1-RARα cKO mice. Bar graphs show quantification of percentage of alternation (left) and number of entries (right). F, Representative image of stereotaxic Cre or mCre injection into V1 of RARαfl/fl mice. Scale bar, 500 μm. G, Quantification of texture preference in V1-RARα cKO mice. Bar graphs show percentage of time spent at texture 400 and 220 (left; **p < 0.01, ***p < 0.001, one-way ANOVA) and discrimination ratios of the two textures of V1-RARα cKO mice (right). H, Quantification of Y-maze test in V1-RARα cKO mice. Bar graphs show quantification of percentage of alternation (left) and number of entries (right). I, J, Schematics (top) and quantification (bottom) of novel texture discrimination in S1-RARα cKO mice with two different texture pairs. Bar graphs show percentage of time spent at each texture during the trial and test phase (bottom left; **p < 0.01, ***p < 0.001, one-way ANOVA) and discrimination ratios between the two textures during the corresponding phase (right; *p < 0.05, **p < 0.01, two-way ANOVA). In all graphs, data represent mean values ± SEM, and n represents the number of animals used for each condition unless otherwise stated.