Figure 6.

Synaptic release of ACh primarily recruits nAChRs in layer 2/3 non-FS neurons. A, Responses to hyperpolarizing and depolarizing current steps in a representative layer 2/3 non-FS neuron. B, Average response in current-clamp from layer 2/3 non-FS cells (n = 4) to 5 ms optical activation (blue bar) followed by a 200 ms puff of muscarine chloride (1 mm; red bar). C, Summary data (n = 4 cells), showing that responses evoked by muscarine were completely blocked by atropine. Shaded areas and error bars denote SEM.