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. 2018 Jun 6;38(23):5415–5428. doi: 10.1523/JNEUROSCI.1653-17.2018

Figure 1.

Figure 1.

ARMS regulates BDNF release in DRG and cortical neurons. A, ARMS depletion in cultured DRG neurons. Cultured DRG neurons were infected at DIV 4 with control (shControl), ARMS shRNA-1 (shARMS-1), or ARMS shRNA-2 (shARMS-2) lentiviruses and lysates were obtained at DIV 11. Western blot analyses were performed. A representative blot is shown (n = 4). B, BDNF secretion in response to NGF is enhanced in ARMS-depleted DRG neurons. BDNF ELISA was performed using the supernatant of DRG neurons from A that were nonstimulated (basal) and then stimulated with NGF for 30 min at DIV 11. Cell lysates were collected to assess BDNF levels (n = 4). Paired Student's t test, mean ± SEM. shControl versus shARMS-1, t = 3.745, df = 3; shControl versus shARMS-2, t = 3.638, df = 3. C, ARMS knockdown in cultured cortical neurons. Cultured cortical neurons were infected with shControl, shARMS-1, or shARMS-2 lentiviruses at DIV 2 and with lentiviruses expressing BDNF at DIV 7. Cell lysates were obtained at DIV 10. Western blot analyses were performed. A representative blot is shown (n = 4). D, ARMS knockdown potentiates BDNF release in response to different stimuli in cortical neurons. BDNF ELISA was performed using the supernatant of cortical neurons from C that were nonstimulated (basal) and then stimulated with KCl, NT-3, or NT-4 for 30 min at DIV 10 (n = 9, n = 5, and n = 4 for shControl, shARMS-1, and shARMS-2, respectively). Unpaired Student's t test, mean ± SEM. K+ Depol: shControl versus shARMS-1, t = 2.846, df = 12; shControl versus shARMS-2, t = 5.923, df = 11; NT-3: shControl versus shARMS-1, t = 3.917, df = 12; shControl versus shARMS-2, t = 10.03, df = 11; NT-4: shControl versus shARMS-1, t = 3.742, df = 12; shControl versus shARMS-2, t = 9.148, df = 11. See also Figure 1-1.