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. 2018 May 21;24:3348–3356. doi: 10.12659/MSM.910350

Table 2.

Sequences of primers and PCR products size of real-time quantitative PCR.

Gene* GenBank accession Forward primer (5′-3′) Reverse primer (5′-3′) Product size (bp)
β-actin NM_007393 AGAGGGAAATCGTGCGTGAC CAATAGTGATGACCTGGCCGT 139
TNF-α NM_013693 CATCTTCTCAAAATTCGAGTGACAA TGGGAGTAGACAAGGTACAACCC 175
IFN-γ NM_008337 TCAAGTGGCATAGATGTGGAAGAA TGGCTCTGCAGGATTTTCATG 92
IL-6 NM_031168 GAGGATACCACTCCCAACAGACC AAGTGCATCATCGTTGTTCATACA 141
IL-10 NM_010548 GGTTGCCAAGCCTTATCGGA ACCTGC CCACTGCCTTGCT 190
iNOS NM_010927 CAGCTGGGCTGTACAAACCTT ATTGGAAGTGAAGCGTTTCG 94
*

TNF-α – tumor necrosis factor alpha; IFN-γ – interferon gamma; IL-6 – interleukin 6; IL-10 – interleukin 10; iNOS – inducible nitric oxide synthase.