Table 2.
Gene* | GenBank accession | Forward primer (5′-3′) | Reverse primer (5′-3′) | Product size (bp) |
---|---|---|---|---|
β-actin | NM_007393 | AGAGGGAAATCGTGCGTGAC | CAATAGTGATGACCTGGCCGT | 139 |
TNF-α | NM_013693 | CATCTTCTCAAAATTCGAGTGACAA | TGGGAGTAGACAAGGTACAACCC | 175 |
IFN-γ | NM_008337 | TCAAGTGGCATAGATGTGGAAGAA | TGGCTCTGCAGGATTTTCATG | 92 |
IL-6 | NM_031168 | GAGGATACCACTCCCAACAGACC | AAGTGCATCATCGTTGTTCATACA | 141 |
IL-10 | NM_010548 | GGTTGCCAAGCCTTATCGGA | ACCTGC CCACTGCCTTGCT | 190 |
iNOS | NM_010927 | CAGCTGGGCTGTACAAACCTT | ATTGGAAGTGAAGCGTTTCG | 94 |
TNF-α – tumor necrosis factor alpha; IFN-γ – interferon gamma; IL-6 – interleukin 6; IL-10 – interleukin 10; iNOS – inducible nitric oxide synthase.