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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Neurotox Res. 2018 May 26;34(3):757–762. doi: 10.1007/s12640-018-9913-3

Figure 3.

Figure 3

Effects of ethanol (EtOH) 500 mM and donepezil (DON 0.1 and 1 μM) alone or in combination on caspase-3 levels in SH-SY5Y cells. DON was applied 2 h before EtOH and cells were incubated for 24 h. Immunnoblots of beta-actin and total caspase-3 are included. Caspase-3 values were normalized per beta-actin protein. Values are mean ± SEM of 4 independent experiments. In every experiment, each sample was run in duplicate. **P<0.01 compared to control, #P<0.05, ##P<0.01 compared to EtOH.