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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Basic Clin Pharmacol Toxicol. 2017 Nov 13;122(2):208–213. doi: 10.1111/bcpt.12875

Fig. 2.

Fig. 2

Effects of post-mortem interval and tissue heat stabilization (HS) on global 5hmC levels in cerebellum. To study the effect of post-mortem interval, the cerebellum from three groups of neonatal and adult animals were kept at room temperature for 0–540 min. before they were snap-frozen (SF) and stored at −80°C. To determine the effect of HS, the cerebellum from three groups of neonatal animals were instantly heat-stabilized and kept at room temperature for 0–540 min. before storage. Values represent mean ± S.E.M. ***p < 0.001, compared with the neonate SF group at respective time-points. #p < 0.05 compared with the adult 0-min. group. No differences were demonstrated between the neonatal SF and HS groups (ANOVA followed by Fisher’s LSD test).