Figure 4.

ASP alleviates oxidative stress in H9c2 cells. H9c2 cells were pretreated with 50 µg/mL ASP for 4 hours, followed by incubation with 300 µM H₂O₂ for 6 hours. (a and b) ROS accumulation was assessed by performing flow cytometric analysis. (c) Decreased MDA content in H9c2 cells by ASP after exposure to H₂O₂. (d) Inhibition of LDH release in H9c2 cells by ASP after exposure to H₂O₂. Data are expressed as mean ± SD (n = 3). #P < 0.05 versus control; *P < 0.05 versus H₂O₂ alone. ASP, Angelica sinensis polysaccharide; H₂O₂, hydrogen peroxide; ROS, reactive oxygen species; MDA, malondialdehyde; LDH, lactate dehydrogenase.