Fig. 6.
LBN Reduces Fos in Orexin Neurons After Cocaine: A) Orexin sampling method: Subregions of the hypothalamic orexinA-containing region are shown in a coronal view, inset shows enlarged view (sampled from 2.8 to 3.2 mm caudal of bregma. LH: lateral hypothalamus, PFA: perifornical area, DMH: dorsomedial hypothalamus. Photomicrographs at bottom show representative examples of orexin neurons (brown soma) with Fos + nuclei in Ctrl (left) and LBN (right) rats. B) Orexin neuron number: Mean (±SEM) orexin + cells in each hypothalamic subregion is shown for Ctrl (black) and LBN rats (orange). C) Orexin neuron Fos: Percentage of orexin neurons per hemisphere/subregion that were Fos+, and D) Non-Orexin Fos: Number of non-orexin Fos + neurons in each hemisphere in control and LBN rats. In panels B–D, dots represent data from individual rats. *p < .05: Ctrl vs. LBN; **p < .001: Ctrl vs. LBN; ˆp < .05: In control rats, difference in % Fos + Orexin neurons in LH vs. DMH or PFA. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)