Fig. 2.

Effects of CSS on splenic T cells at day 16 assessed using flow cytometry (Expt 1). Spleens were processed from 6 CSS and 6 CON mice (same mice as in Fig. 1). A) FACS dot plots for gating of CD4⁺ and CD8⁺ T cells in representative CON and CSS mice. B) Cell counts for CD4⁺ T cells. C) Cell counts for CD8⁺ T cells. D) FACS dot plots for gating of Th17 cells (CD4⁺/IL-17A⁺) in representative CON and CSS mice. E) Cell counts for Th17 cells. In B), C) and E) the data point for one CSS mouse was excluded because of a methodological problem with the intracellular staining. p value was obtained in an unpaired t-test. CD4, CD8, cluster of differentiation 4, 8; IL-17A, interleukin-17A; Th17, T helper 17.