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. 2018 May 24;14(5):e1007426. doi: 10.1371/journal.pgen.1007426

Fig 4. Increasing RctB concentration promotes Chr2 replication in Chr1 replication-blocked cells.

Fig 4

(A) Time-lapse microscopy of Chr1 replication-blocked cells with additional RctB at low (CVC3052) or high (CVC3125) levels supplied from plasmid prctBLOW or prctBHIGH. Increasing RctB using either plasmid allowed Chr2 replication independent of Chr1 replication. Scale bars, 2 μm. (B) Histogram of ori2 foci number per cell in the presence of low or high RctB, 150 min after addition of 0.2% arabinose. Percentage of cells with one ori1 focus and ≥2 ori2 foci (yellow + blue bar) increased from 7.9 ± 0.9% (Σn = 498) in cells with vector (CVC3145) to 22 ± 1.9% (Σn = 622) in cells with prctBLOW (CVC3052) and to 46 ± 3.5% (Σn = 516) in cells with prctBHIGH (CVC3125). Data represent mean ± SEM of percentages calculated from three biological replicates with at least 100 cells in each replicate. Statistical significance was calculated using a Student’s t-test.