Fig. 5.

Improvement of odd chain fatty acid production of JMY3776 by fed-batch co-feeding of propionate and glucose. a Increase of the accumulation of odd lipids by co-feeding of propionate and glucose. Cells were grown for 16 h in glucose media (YNBD1), then pulses of glucose + propionate (4 and 0.5 g/L, respectively) were added at t = 16, 23, 40, 47 h. The results presented are the mean values for three independent biological replicates. b Microscope image of cells (DIC) and lipid body visualization with Bodipy of JMY2900 (WT), JMY3350 (WT ∆phd1) and JMY3776 (obese ∆phd1). c Increase of odd lipids accumulation by pathway engineering in fed-batch co-feeding cultures