Table 1.

E. coli and Y. lipolytica strains used in this study

Name	Relevant genotype/plasmid description	Source of references
E. coli strains
DH5α	$\Phi$80lacZ∆m15 ∆(lacZYA-argF) U169 recA1 endA1 hsdR17 (r−k, m+k) phoA supE44 thi-1 gyrA96 relA1 λ−	Promega
JME740	DH5α pKS-PUT phd1	[32]
JME547	DH5α pUB4-CRE1	[53]
JME1000	DH5α pKS-PLT tgl4	[37]
JME1077	DH5α pGEMT easy-PUT mfe1	[36]
JME1128	DH5α JMP62-pTEF-GPD1-URA3ex	[36]
JME1822	DH5α JMP62-pTEF-DGA2-LEU2ex	[38]
Y. lipolytica strains
Po1d (JMY195)	MATa ura3-302 leu2-270 xpr2-322	[30]
JMY2900	Po1d Ura+ Leu+	[31]
JMY1203	Po1d phd1::URA3ex	[32]
JMY3279	Po1d ∆phd1	This study
JMY3348	Po1d ∆phd1 mfe1::URA3ex	This study
JMY3350	Po1d phd1::URA3ex + LEU2	This study
JMY3396	Po1d ∆phd1 mfe1::URA3ex tgl4::LEU2ex	This study
JMY3433	Po1d ∆phd1 ∆mfe1 ∆tgl4	This study
JMY3576	Po1d ∆phd1 ∆mfe1 ∆tgl4 +pTEF-DGA2-LEU2ex	This study
JMY3776	Po1d ∆phd1 ∆mfe1 ∆tgl4 +pTEF-DGA2-LEU2ex + pTEF-GPD1-URA3ex	This study