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. 2018 Jun 7;18:110. doi: 10.1186/s12870-018-1325-2

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Localization of the chromosome 9-specific oligo library, 45S rDNA and CentO probes on the chromosomes of O. eichingeri and a triploid (AAC). The chromosome 9-specific (red) and 45S rDNA probes (green) fluorescing in prometaphase chromosomes of the (a) O. eichingeri (CC, 2n = 2× = 24) and (c) the triploid (AAC, 2n = 3× = 36) derived from O. eichingeri and 02428. b and d The chromosome 9-specific and 45S rDNA signals were digitally separated from the rest of the chromosomes in panels (a) and (c), respectively. e The chromosome 9-specific (red) and CentO (green) probes on chromosomes of the triploid. f The chromosome 9-specific and CentO signals were digitally separated from the rest of the chromosomes in (e). White arrows indicate the chromosome 9 from O. eichingeri. Chromosomes were stained with DAPI. Bars, 5 μm