Figure 1.

Plasmid-based sgRNA constructs as a tool for a genetic perturbation screen in human cells. (A,B) HeLa cells were transfected with sgRNA-expressing plasmids (Control, CXADR, and PI4KB) and infected with CVB3 at an MOI of 5. At 8 h post-infection, cells were fixed and stained with anti-3C antibody (green) and DAPI (blue). (A) Quantification of infection. Mean ± SEM for quadruplicate experiments. (**) P < 0.01. (B) Representative images of infection are shown. (C) Mutation frequencies determined after transient transfection of 35 sgRNAs and Cas9 plasmid.