Table 6.

Primer sequences designed to amplify the 10 most polymorphic Lecythidaceae plastome regions, as sorted by decreasing nucleotide diversity

Window in the alignment	π	Region	Forward primer sequence (5′–3′)	Reverse primer sequence (5′–3′)	Length (bp)a
144,103–145,487	0.04691	ycf1(1)	AGAACCTTTGATTATGTCTCGACG	AGAGACATGCTATAAAAATAGCCCA	1186
95,034–95,741	0.03446	rpl16‐rps3	AGAGTTTCTTCTCATCCAGCTCC	GCTTAGTGTGTGACTCGTTGG	1014
35,585–36,413	0.02920	psbM‐trnD	CCGTTCTTTCTTTTCTATAACCTACCC	ACGCTGGTTCAAATCCAGCT	1093
143,235–144,102	0.02733	ycf1(2)	TGATTCGAATCTTTTAGCATTAKAACT	KCGTCGAGACATAATCAAAGGT	1189
131,180–132,054	0.02576	ccsA‐ndhD	CCGAGTGGTTAATAATGCACGT	GCTTCTCTTGCATTACCGGG	1180
44,398–45,132	0.02537	trnG‐psaB	TCGATYCCCGCTATCCGCC	GCCAATTTGATTCGATGGAGAGA	883
89,032–89,688	0.02464	petD‐rpoA	TGGGAGTGTGTGACTTGAACT	TGACCCATCCCTTTAGCCAA	824
43,412–44,397	0.02456	psbZ‐trnfM	TCCAATTGRCTGTTTTTGCATTAATTG	CCTTGAGGTCACGGGTTCAA	706
37,444–38,345	0.02409	trnE‐trnT	AGACGATGGGGGCATACTTG	CCACTTACTTTTTCTTTTGTTTGTTGA	1324
38,346–40,085	0.02391	trnT‐psbD	GGCGTAAGTCATCGGTTCAA	CCCAAAGCGAAATAGGCACA	1717

π = nucleotide diversity.

^aThe product size (length) references the Bertholletia excelsa plastome.