Table 3.

Quantitative proteomic analysis of functionally relevant red blood cell proteins

Accession	Description	Gene ID	Coverage	No. of Peptides	No. of PSMs	No. of unique Peptides	Average abundance ratio 5×KO:UT control
P02549	Spectrin alpha chain, erythrocytic 1	SPTA1	61	136	599	136	0.96
P11277	Spectrin beta chain, erythrocytic	SPTB	65	128	532	4	0.96
P16157	Ankyrin‐1	ANK1	51	77	347	77	1.02
P35611	Alpha‐adducin	ADD1	39	21	55	20	1.01
P35612	Beta‐adducin	ADD2	50	31	73	19	1.28
P11171	Protein 4.1	EPB41	45	35	178	16	1.02
P16452	Erythrocyte membrane protein band 4.2	EPB42	44	27	89	27	1
Q00013	55 kDa erythrocyte membrane protein	MPP1	55	21	52	21	1.2
Q08495	Dematin	DMTN	51	20	57	20	1
P28289	Tropomodulin‐1	TMOD1	47	15	32	15	1.03
B4DW52	Beta actin	ACTB	55	14	152	1	0.86
E2RVJ0	Anion exchanger 1 (band 3)	SLC4A1	39	29	267	29	1.04
A0A0C4DFT7	Glycophorin‐A	GYPA	37	5	19	5	1
P04921	Glycophorin‐C	GYPC	27	2	31	2	0.99
P27105	Erythrocyte band 7 integral membrane protein	STOM	52	14	102	14	1.35
E7EMK3	Flotillin‐2	FLOT2	35	15	32	15	0.98
Q59GX2	Solute carrier family 2 (Facilitated glucose transporter) Glut1	SLC2A1	13	9	86	9	1.14
C8C504	Beta‐globin	HBB	94	21	2,705	1	0.93
P00915	Carbonic anhydrase 1	CA1	72	16	370	2	1.27
H7BY55	Complement decay‐accelerating factor	CD55	9	6	12	6	1.55
E9PR17	CD59 glycoprotein	CD59	23	3	8	3	1.44
P35613	Basigin	BSG	36	10	22	10	1.23
A0A068W6W9	Urea transporter	SLC14A1	5	2	2	2	0.94
A0A068W6H0	Basal cell adhesion molecule (Lutheran blood group)	BCAM	8	4	4	4	0.69
P51811	Membrane transport protein XK	XK	11	5	9	5	0.63
Q08722	Leukocyte surface antigen CD47	CD47	6	2	4	2	0.61
P16070	CD44 antigen	CD44	3	3	5	3	0.51
Q14773	Intercellular adhesion molecule 4	ICAM4	14	3	4	3	0.07
B2LR44	Blood group Rh(D) polypeptide	RHD	4	2	6	2	0.02

Table listing relative abundance of selected blood group, membrane and cytoskeletal proteins relevant to red blood cell immunogenicity and structural integrity based on data set summarised in Fig EV4.