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. 2018 May 16;10(6):e8289. doi: 10.15252/emmm.201708289

Figure 5. MYC activation and T‐025 treatment synergistically induced apoptosis.

Figure 5

  • A, B
    Dox treatment‐induced/activated MYC in MYC‐inducible SK‐MEL‐28 cells. SK‐MEL‐28 cells were treated with 0.5 μg/ml Dox for 54 h, and MYC mRNA level was analyzed with RT–PCR (A). E‐Box reporter activities of SK‐MEL‐28 cells after 48 h of Dox treatment were measured as MYC transcriptional activities.
  • C
    Dose–response growth inhibition curve of T‐025 in MYC‐inducible SK‐MEL‐28 cells. SK‐MEL‐28 cells pretreated with Dox for 48 h were additionally incubated with T‐025 for 72 h.
  • D, E
    Apoptosis induction caused by T‐025 in MYC‐inducible SK‐MEL‐28 cells. SK‐MEL‐28 cells pretreated with Dox for 48 h were additionally treated with T‐025 for 24 h, and then, apoptosis was measured by caspase‐3/7 activity (D) or immunoblotting of cPARP1 (E).
  • F
    4OTH treatment‐induced/activated MYC in MYC‐inducible U2OS cells. E‐Box reporter activities of U2OS cells after 48 h of 4OTH (500 nmol/l) treatment were measured as MYC transcriptional activities.
  • G
    Dose–response growth inhibition curve of T‐025 in MYC‐inducible U2OS cells. U2OS cells pretreated with 4OTH for 48 h were additionally incubated with T‐025 for 72 h.
  • H
    Apoptosis induction caused by T‐025 in MYC‐inducible U2OS cells. U2OS cells pretreated with 4OTH for 48 h were additionally treated with T‐025 for 24 h, and then, apoptosis was measured by immunoblotting of cPARP1.
  • I
    Dose–response growth inhibition curve of T‐025 in SK‐BR3 or MCF7 cells transfected with MYC or DYRK1A siRNA. The cells pretreated with siRNA for 24 h were additionally incubated with T‐025 for 72 h.
Data information: In (A, B and F), an unpaired Student's t‐test or an unpaired Student's t‐test with Welch's correction was performed. In (D), Tukey's test was performed. In (A–D, F, G and I), data are shown as the means ± s.d. of three independent experiments (n = 3). IC50 values and 95% confidence intervals (95% CIs) in (C, G, and I) are described in Appendix Fig S8.Source data are available online for this figure.