Figure 6. Ectopic MYC activation altered pre‐mRNA splicing without transcriptional regulation of CLK.

• A
  MYC induction increased expression of SRSF1 and PRMT5, but not CLK family kinases. The same samples of Fig 5A were analyzed with RT–PCR.
• B
  Changes in CPM values of CLK families, PRMT5, and other splicing‐related genes calculated from RNA‐Seqs data of MYC‐inducible SK‐MEl‐28 cells treated with Dox for 54 h.
• C
  The numbers of AS events modulated by Dox with a BF > 20 and |ΔPSI| > 0.1 were counted and categorized according to the AS type.
• D
  Comparison between MYC induction‐dependent and CLK inhibitor‐dependent alternative splicing events.
• E
  Effects of T‐025 on MYC‐inducible SK‐MEL‐28 cells were analyzed by immunoblotting. SK‐MEl‐28 cells pretreated with Dox for 48 h were additionally treated with T‐025 for 24 h.
• F, G
  Exon 3 skipping of NOP16 was induced by T‐25. MYC‐inducible SK‐MEL‐28 cells pretreated with Dox were treated with 100 nmol/l T‐025 for 6 h. NOP16 exon 2–3 and 2–4 transcripts were measured by quantitative RT–PCR, and the PSI value of each sample was calculated (F). The copies of exon 2–3 transcripts are shown (G).

Data information: In (A), an unpaired Student's t‐test was performed. In (F and G), Tukey's test was performed. In (A, F, and G), data are shown as the means ± s.d. of three independent experiments (n = 3).Source data are available online for this figure.