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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: J Tissue Eng Regen Med. 2017 Nov 28;12(3):e1623–e1635. doi: 10.1002/term.2589

FIGURE 4.

FIGURE 4

Proliferation and gene expression of induced pluripotent stem cell (iPSC)-derived progenitor cells in repopulated human lung matrix. (a) Immunostaining for proliferating cell nuclear antigen (PCNA; red), caspase (green), and 4′,6-diamidino-2-phenylindole (DAPI) in an engineered human lung at day 4 (scale bar = 40 µm) and (b) quantification of percentage of PCNA+ nuclei (y axis, percentage proliferation based on the number of positive nuclei stained for PCNA compared with total cell numbers in three high power fields). (c) Quantitative reverse transcription-PCR analysis of epithelial markers in recellularized lung tissue with iPSC-lung progenitor cells following 4 days of culture. The expression of lung alveolar and airway epithelial cell markers are normalized to glyceraldehyde-3-phosphate dehydrogenase levels, and relative expression of each gene was calculated to normal human lung tissue. (n = 3 tissue samples analysed/lung for quantitative reverse transcription-PCR and error bars indicate ± SEM, *indicates p < .05 and ns indicates no significant difference). CCSP = Clara cell secretory protein; FOXJ1 = Forkhead box protein J1; PCNA = proliferating cell nuclear antigen; SPC = surfactant protein C