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. Author manuscript; available in PMC: 2019 Jun 1.
Published in final edited form as: J Mol Cell Cardiol. 2018 Apr 19;119:51–63. doi: 10.1016/j.yjmcc.2018.04.011

Figure 9.

Figure 9

RAW264.7 macrophages were treated with medium alone (CTRL), LPS (100ng/ml), IL-4 (30ng/ml), IL-10 (20ng/ml) treated with the pan HDAC inhibitor TSA (100nM) or SAHA (5μM) +/−LPS (100ng/ml) and incubated for 6 hours after treatment. A) Cells were isolated 6 h after treatment and qRT-PCR analysis was performed on mRNA for MMP-9 and normalized to GAPDH. The fold change mRNA values are shown as fold change of medium alone cells or cells treated with LPS. B) Representative zymogram and quantification of the abundance of the active form of MMP-9 (86 kDa) secreted from RAW264.7 cells after indicated treatment for 12 hr. For all the data shown in A and B, each bar represents the fold change ± SEM of three independent experiments. #p<0.05 vs control, *p<0.05 vs control + LPS.