Fig 7. Spot 42 interacts with the downstream part of the hilD 3’UTR.

(A) EMSA assay using 4 nM of hilD 3’UTR RNA radiolabeled incubated with increasing concentration of either Spot 42 or Spot 42^mut2 RNA (0, 280, 560, 1700 nM). (B) Schematic representation of the hilD 3’UTR and the UTR^L and UTR^R fragments. EMSA using 4 nM of radiolabeled Spot 42^WT (C) or Spot 42^mut2 (D) incubated with increasing concentrations (0, 56, 280, 560, 1,700 nM) of UTR^L or UTR^R. All RNA transcripts used were obtained by T7 in vitro transcription. Samples were subjected to electrophoresis in a native gel and band shifts were observed upon drying and exposure of the gel.