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. 2018 Jun 7;16(6):e2005069. doi: 10.1371/journal.pbio.2005069

Fig 6. Regulation of sperm activation by ZIPT-7.1 is conserved in nematodes.

Fig 6

(A) Maximum likelihood phylogeny of all ZIP7 homologs identified in eight nematode species: Caenorhabditis briggsae (Cbr), C. nigoni (Cni), C. remanei (Cre), C. tropicalis (Ctr), C. brenneri (Cbn), C. elegans (Cel), C. japonica (Cja), and Onchocerca volvulus (Ovo). Sequences were obtained from wormbase.org and aligned using ClustalX, and calculations were done using PhyML with 100 bootstrap replications. Blue indicates the ZIPT-7.1 subfamily and green the ZIPT-7.2 subfamily. (B) C. tropicalis zipt-7.1 mutations produced by gene editing. Gray numbers indicate positions in the coding sequence of the gene. Deleted nucleotides are shown as dashes, and inserted nucleotides are blue. We selected the frameshift alleles v332 and v334 as representative null alleles and the in-frame deletion v335 as a nearly wild-type control. (C, D) Brood sizes of ctr-zipt-7.1 hermaphrodites and males, presented as in Fig 2. JU1373 is the wild-type strain of C. tropicalis. For D–F, wild-type males were him-8(v287) and mutant males were him-8(v287); zipt-7.1(v332). (E) Photomicrographs of spermatids stained with Zinpyr-1 to reveal labile zinc levels, as in Fig 5A. Scale bars are 5 μm. (F) Quantitation of fluorescence intensity, as in Fig 5B. The individual numerical values for panels C, D, and F can be found in S1 Data. Cbn, C. brenneri; Cbr, C. briggsae; Cel, C. elegans; Cja, C. japonica; Cni, C. nigoni; Cre, C. remanei; Ctr, C. tropicalis; Ovo, Onchocerca volvulus.