Table 1. Compound potency in biochemical kinase assay and p-PTK6 in-cell ELISA assay in engineered HEK293T cells overexpressing PTK6 WT.

Compound (type of inhibitor)	Biochemical assay IC50 (uM)	p-PTK6 in-cell ELISA IC50 (uM)
21c (Type I)	0.056 ± 0.004	0.063 ± 0.018
21a (Type I)	0.025 ± 0.003	0.023 ± 0.011
PF-6683324 (Type II)	0.076 ± 0.001	0.70 ± 0.33
PF-6698840 (Type II)	0.054 ± 0.001	0.25 ± 0.10
PF-6737007 (negative control)	>10	>10

The IC₅₀ values are listed as the mean ± standard deviation of at least four independent experiments.