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. 2018 Jun 7;13(6):e0198795. doi: 10.1371/journal.pone.0198795

Fig 2. Identification of BRAF mutations in ultra-short DNA.

Fig 2

A and B. Detection of BRAF V600E1 mutation (A) and V600K mutation (B) in 45-, 35-, or 25-base synthesized oligonucleotides (S1 Table), which mimic severely fragmented single-stranded DNA. C. Cross-reactivity with non-target mutations, i.e. BRAF V600A, V600D, V600G, V600M, and V600R, presented in 45-base oligonucleotide templates (S1 Table). Allele-specific primers for BRAF V600E and V600K were combined in the same quantitative PCR reaction.