Table 3. Characteristics of BRAF V600E mutation-positive cases in Thai colorectal cancer tissues.
| Sample number | Age | Sex | Organ | Tumor percentage | qPCR dCt | BRAF genotype by GeneRead QIAact Actionable Insights Tumor Panel | |
|---|---|---|---|---|---|---|---|
| 200-bp assay | 41-bp assay | ||||||
| 1 | 58 | F | Urinary bladder | NA | 11.53 | 2.12 | c.1799T>A mutation not detected |
| 2 | 63 | M | Colon | NA | 6.26 | 1.94 | c.1799T>A mutation (16.5%) |
| 3 | 69 | M | Sigmoid colon | 80 | 4.02 | 0.71 | c.1799T>A mutation (18.6%) |
| 4 | 70 | M | Colon | 90 | 4.96 | 2.36 | c.1799T>A mutation (43.0%) |
| 5 | 28 | F | Colon | 50 | 9.76 | 3.55 | c.1799T>A mutation (19.5%) |
| 6 | 63 | M | Ilium | 70 | 2.72 | 2.79 | c.1799T>A mutation (28.9%) |
(NA = not available, dCt = Ct sample−Ct control in the same run)