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. 2018 May 25;14(5):e1007412. doi: 10.1371/journal.pgen.1007412

Fig 8. m6A-dependent rescue of alternative splicing defects in Ythdc1-deficient oocytes.

Fig 8

Postnatal day 12 Ythdc1fl/- Ddx4-Cre (cKO) oocytes were injected with mRNAs encoding wild-type or m6A-binding-deficient mutant (W377A W428A) YTHDC1 as marked on top of the gel panel, followed by RT-PCR analysis of LSVs. Left panel, schematic illustration of alternative splicing events for each transcript that correspond to the PCR products shown in the center panel. Each rectangle represents one exon, and exons subject to alternative splicing are marked red. Right panel, plot depicting quantification of ratios of band intensity or a single band intensity, with the value for wild-type oocyte (lane 1) set at 1. Asterisks indicate bands used for quantification. Enpp5 and Parp6: ratio of the upper band / the lower band; Tmem2: ratio of the lower band / the upper band. Actb serves as a loading control.