Fig 2.

ITS1-PCR gene sequence alignment of L. tropica isolates using the Clustal Omega multiple sequence alignment program. Matching residues are highlighted with the same color. Gaps are denoted as dashes. “*” indicates positions which have a single, fully conserved residue. “:” indicates that a ‘strong’ group is fully conserved. “.” indicates that a ‘weaker’ group is fully conserved. These are all the positively scoring groups that occur in the Gonnet Pam250 matrix. The strong and weak groups are defined as strong score > 0.5 and weak score = < 0.5 respectively.